Rpmi 1640, also known as rpmi medium, is a growth medium used in cell culture. Up to now, such media were not autoclavable because of. Without lglutamine and sodium bicarbonate, autoclavable. Roswell park memorial institute rpmi 1640 medium has since been found. Rpmi 1640 medium was originally developed to culture human. G708a application of the substance the mixturefor research use only details of the supplier of the safety data sheet manufacturersupplier. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of co 2. Rpmi1640 medium has been used for the culture of human normal and neoplastic leukocytes. Rpmi1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72hour phytohemagglutinin pha stimulation assay. Rpmi1640 medium with lglutamine, without sodium bicarbonate.
Rpmi1640 medium with lglutamine, without sodium bicarbonate, powder, suitable for cell culture. The formulation is based on the rpmi1630 series of media utilizing a. Rpmi 1640 powder medium with lglutamine, with 25 mm hepes, wo sodium bicarbonate. Moore, former director of roswell park memorial institute.
The medium was developed for culture of normal and neoplastic leukocytes, but also marrow cells and hybridoma cells. Rpmi1640 medium modified to contain 2 mm lglutamine, 10 mm hepes, 1 mm sodium pyruvate, 4500 mgl glucose, and 1500 mgl sodium bicarbonate, for use in incubators using 5% co 2 in air. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. Rpmi 1640 medium contains biotin, vitamin b 12, and paba, which are not found in eagles minimal essential medium or dulbeccos modified eagle medium. Rpmi1640 media rpmi1640 medium was developed by moore et al. Rpmi1640 medium has been used for the culture of normal and neoplastic leukocytes. With lglutamine and without sodium bicarbonate product number r6504 product description. Aggravation of coldinduced injury in verob4 cells by rpmi 1640. The formulation is based on the rpmi1630 series of media utilizing a bicarbonate buffering system. Rpmi 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. We offer a variety of rpmi 1640 medium modifications for a range of cell culture applications. Powder mixture to prepare rpmi 1640 medium according to moore, g. Rpmi 1640 is a medium for suspension culture investigated by george e.
Rpmi is a commonly used cell culture media for diagnostic virology 1, 2. Cold storage in rpmi 1640 medium, a recommended cell culture medium for verob4 cells, surprisingly, strongly enhanced coldinduced cell. Rpmi 1640 medium has been used for the culture of human normal and neoplastic leukocytes. Ccf media production unit vendor product alternatives ucsf.
Roswell park memorial institute rpmi 1640 medium has since been found suitable for a variety of mammalian cells, including hela, jurkat, mcf7, pc12, pbmc, astrocytes, and carcinomas. Shop online for a wide selection of gibco rpmi 1640 medium originally developed to. Rpmi 1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin pha stimulation assay. Rpmi 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
362 95 336 949 416 282 893 1287 859 126 1249 778 1192 1544 857 549 1493 666 115 1349 785 1425 643 1511 675 860 895 592 27 1076 380 46 678 1503 108 450 803 1268 284 188 163 707 1362 580 486 1041 337 684